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Harness the power of HRM using dedicated QIAGEN HRM Kits for applications such as genotyping (see figure ' for data from the Type-it HRM PCR Kit), quantitative methylation analysis (see figure ' for data from the EpiTect HRM PCR Kit), gene scanning, and sequence matching. The Rotor-Gene Q is the only real-time cycler currently capable of deciphering the most difficult class IV SNPs by HRM. The average C T value was 24.94 with a standard deviation of only 0.05, equivalent to a CV of 0.2%. Human genomic DNA was used as template in 72 replicate real-time PCRs using a self-designed TaqMan® assay for BCL2 on the Rotor-Gene Q without ROX normalization. The average difference in the C T values between all dilutions was 1.07 cycles. Five replicate reactions were run for each dilution using a self-designed TaqMan® assay for IL1R2 and the Rotor-Gene Probe PCR kit on the Rotor-Gene Q. Twofold dilutions of human genomic DNA from 30 ng (10,000 copies) to 0.06 ng (20 copies) were used as a template in real-time PCR. All pseudo-unknowns were correctly clustered according to genotype. A/T polymorphisms (class IV SNPs) are most difficult to discriminate due to minute differences between homozygote alleles (in this example, less than 0.1☌). Data analysis was performed with the unsupervised mode of Rotor-Gene ScreenClust HRM Software. A human A/T SNP in the AHRR7 gene was analyzed using genomic DNA from wild-type (blue), homozygous mutant (green), and heterozygous (red) samples.Įxperiments were performed using the Type-it HRM PCR Kit and a Rotor-Gene Q cycler with an HRM channel. Explore the to learn more about the Rotor-Gene Q.
Rotor gene 6000 software software#
Is the new operating and analysis software for life science qPCR applications, with a plug-in concept that lets you add new functionality without affecting established workflows. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications. QIAGEN's real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands.
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We do not provide any download link points to Rapidshare, Depositfiles, Mediafire, Filefactory, etc. The same high efficiency, enabling reliable relative quantification of gene expression. The comprehensive Rotor-Gene Q software package supports all current state-of-the art real-time analysis procedures from.
Rotor gene 6000 software series#
Open the Rotor-Gene Q Series Software 2.0 on the desktop of the computer that is connected to the. Set up a PCR protocol template file on the Rotor-Gene Q Series software as follows: 1. The download file hosted at publisher website.
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